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A simple microplastic splitter for subsampling expanded polystyrene particles.

Ryota Nakajima1, Noriyuki Isobe1, Nisha Singh1

  • 1Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima Yokosuka, Kanagawa, Japan.

Methodsx
|December 6, 2023
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Summary

A new radial splitter precisely divides buoyant microplastics, like expanded polystyrene (EPS), from marine samples. This simple stainless steel device overcomes accuracy issues of traditional plankton splitters for accurate microplastic analysis.

Keywords:
DividingEPSMicroplasticsPlastic particlesRadial microplastic splitterSplitting

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Area of Science:

  • Environmental Science
  • Marine Biology
  • Analytical Chemistry

Background:

  • Microplastic analysis requires subsampling due to large sample volumes from net hauls.
  • Traditional plankton splitters (Folsom, Motoda) inaccurately separate highly buoyant microplastics like expanded polystyrene (EPS).
  • Buoyant microplastics adhere to the inner walls of conventional splitting devices, compromising sample integrity.

Purpose of the Study:

  • To develop a novel, accurate method for subsampling buoyant microplastics.
  • To address the limitations of existing plankton splitters for EPS and similar materials.
  • To create a versatile tool for precise microplastic sample division.

Main Methods:

  • A simple radial splitter, inspired by an apple cutter, was designed and constructed from stainless steel.
  • The radial splitter was used to divide marine microplastic samples, specifically focusing on expanded polystyrene (EPS).
  • EPS microplastic samples were uniformly partitioned into eight aliquots for analysis.

Main Results:

  • The developed radial splitter efficiently and uniformly divided EPS microplastic samples into eight aliquots.
  • No significant differences were observed between the divided aliquots, indicating high accuracy.
  • The device proved effective for partitioning other buoyant microplastics, including polypropylene and polyethylene.

Conclusions:

  • The novel radial splitter provides a precise and reliable method for subsampling buoyant microplastics.
  • This tool enhances the accuracy of microplastic enumeration and analysis, particularly for challenging materials like EPS.
  • The device offers a versatile solution for marine microplastic research, improving sample processing efficiency.