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Related Experiment Video

Updated: Jul 9, 2025

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
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Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal

Stphannie Jamyla de Araújo Barbosa1, Amanda Silveira da Silva2, Maisie Mitchele Barbosa Oliveira3

  • 1Universidade Federal do Rio Grande do Norte - Postgraduate Program in Pharmaceutical Science - Natal (RN), Brazil.

Acta Cirurgica Brasileira
|December 6, 2023
PubMed
Summary

Lacticaseibacillus casei (L. casei) oral use prevented intestinal mucositis damage in mice treated with 5-fluorouracil. This probiotic also reduced specific long-chain fatty acids in the feces, indicating a protective effect on gut health.

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Area of Science:

  • Gastroenterology and Probiotic Research
  • Experimental models of chemotherapy-induced toxicity

Background:

  • Intestinal mucositis is a debilitating side effect of chemotherapy, particularly 5-fluorouracil (5-FU).
  • Alterations in fecal long-chain fatty acids (LCFAs) may indicate gut barrier dysfunction.
  • Probiotics, such as Lacticaseibacillus casei (L. casei), are explored for their potential to mitigate chemotherapy-induced side effects.

Purpose of the Study:

  • To investigate the prophylactic effects of oral L. casei on 5-FU-induced intestinal mucositis.
  • To analyze the levels of LCFAs and other compounds in fecal samples following L. casei administration.

Main Methods:

  • An experimental model using Swiss mice subjected to 5-FU treatment to induce intestinal mucositis.
  • Groups included negative control (saline), positive control (5-FU), and L. casei treatment group (L. casei + 5-FU).
  • Fecal samples were analyzed for LCFAs and sterols using gas chromatography-mass spectrometry (GC-MS).
  • Histopathological analysis of intestinal tissues was performed.

Main Results:

  • L. casei administration preserved the intestinal mucosal epithelium and glands, reducing necrosis signs in 5-FU treated mice.
  • Fecal analysis revealed significant reductions in linoleic acid (18:2) and oleic acid (18:1) concentrations post-L. casei treatment.
  • Goblet cell numbers were decreased in the L. casei group, suggesting altered mucus production.

Conclusions:

  • Prophylactic oral administration of L. casei effectively prevented 5-FU-induced intestinal mucosal damage.
  • L. casei treatment modulated fecal LCFA levels, correlating with its protective effect against mucositis.
  • These findings highlight L. casei's potential as a therapeutic agent for managing chemotherapy-induced gastrointestinal toxicity.