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Hymenobacter endophyticus sp. nov., isolated from wheat leaf tissue
Xue Zhang1, Xue-Mei Duan2,3, Jin Cheng1
1College of Animal Science and Technology, Hebei Normal University of Science &Technology, Qinhuangdao 066600, PR China.
International Journal of Systematic and Evolutionary Microbiology
|December 7, 2023
Summary
A novel bacterium, Hymenobacter endophyticus sp. nov., was discovered in wheat leaf tissues. This aerobic, Gram-negative bacterium represents a new species within the Hymenobacter genus, expanding our understanding of plant-associated microbes.
Area of Science:
- Microbiology
- Plant Pathology
- Bacteriology
Background:
- The genus Hymenobacter comprises diverse bacteria, often isolated from various environmental sources.
- Understanding bacterial diversity in plant tissues is crucial for plant health and agricultural applications.
Purpose of the Study:
- To characterize a novel bacterium isolated from wheat leaf tissues.
- To determine the taxonomic position of the novel isolate within the Hymenobacter genus.
- To propose a new species name for the identified bacterium.
Main Methods:
- Isolation and cultivation of bacteria from wheat (Triticum aestivum) leaf tissues.
- Phylogenetic analysis using 16S rRNA gene sequences.
- Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) calculations.
- Biochemical and chemotaxonomic analyses, including fatty acid profiling and G+C content determination.
Main Results:
- Strain ZK17L-C2^T, isolated from wheat, is an aerobic, non-motile, Gram-negative, rod-shaped bacterium.
- Phylogenetic analysis placed strain ZK17L-C2^T within the genus Hymenobacter, closely related to H. rigui and H. metallilatus.
- Low dDDH (26.6-26.5%) and ANI (84.9-84.8%) values confirmed its distinctiveness from known species.
- The bacterium exhibits specific growth conditions (pH 5.5-7.5, 15-30°C) and possesses characteristic major fatty acids (iso-C15:0, C16:0, C18:0).
Conclusions:
- Strain ZK17L-C2^T represents a new species of the genus Hymenobacter.
- The proposed name for this novel species is Hymenobacter endophyticus sp. nov.
- This discovery contributes to the knowledge of endophytic bacteria associated with agricultural crops.
