Multi-Enzyme-Based Superabsorbent Hydrogel for Self-Enhanced NIR-II Photothermal-Catalytic Antibacterial Therapy

The synergistic strategy of nanozyme-based catalytic therapy and photothermal therapy holds great potential for combating bacterial infection. However, challenges such as single and limited enzyme catalytic property, unfavorable catalytic environment, ineffective interaction between nanozymes and bacteria, unsafe laser irradiation ranges, and failed trauma fluid management impede their antibacterial capability and wound healing speed. Herein, for the first time, a PNMn hydrogel is fabricated with multi-enzyme activities and excellent near-infrared (NIR)-II photothermal performance for self-enhanced NIR-II photothermal-catalytic capabilities to efficiently eradicate bacteria. This hydrogel triggers parallel and cascade reactions to generate •OH, •O₂ ^-, and ¹O₂ radicals from H₂O₂ and O₂ without external energy input. Notably, it provides a suitable catalytic environment while capturing bacteria (≈30.1% of Escherichia coli and ≈29.3% of Staphylococcus aureus) to reinforce antibacterial activity. Furthermore, the PNMn hydrogel expedites skin wound healing by managing excess fluid (swelling rate up to ≈7299%). The PNMn hydrogel possesses remarkable stretching, elasticity, toughness, and adhesive characteristics under any shape of the wound, thus making it suitable for wound dressing. Therefore, the PNMn hydrogel has great potential to be employed as a next-generation wound dressing in the clinical context, providing a non-antibiotic strategy to improve the antibacterial performance and promote wound healing.