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Regulation of Dihydropyrimidinase-like 3 Gene Expression by MicroRNAs in PC12 Cells with Induced Ischaemia and
Kisang Kwon1, Ji-Hye Song2, Hyewon Park3
1Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, South Korea.
Abstract:
Although hypothermic treatment has been reported to have some beneficial effects on ischaemia at the clinical level, the mechanism of ischaemia suppression by hypothermia remains unclear due to a lack of mechanism understanding and insufficient data. The aim of this study was to isolate and characterize microRNAs specifically expressed in ischaemia-hypothermia for the dihydropyrimidinase-like 3 (Dpysl3) gene. PC12 cells were induced with CoCl2 for chemical ischaemia and incubated at 32 ℃ for hypothermia. In ischaemia-hypothermia, four types of microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, and miR-497-5p) were highly related to the Dpysl3 gene based on exosomal microRNA analysis. Dpysl3 gene expression was up-regulated by miR-497-5p but down-regulated by miR-106b-5p, miR-194-5p and miR-326-5p. Our results suggest that these four microRNAs are involved in the regulation of Dpysl3 gene expression. These findings provide valuable clues that exosomal microRNAs could be used as therapeutic targets for effective treatment of ischaemia.
Insights
Hypothermia aids ischemia recovery by altering specific microRNAs that regulate the Dpysl3 gene. These exosomal microRNAs offer potential therapeutic targets for ischemia treatment.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Hypothermic treatment shows clinical benefits for ischemia, but underlying mechanisms remain unclear.
- Understanding the molecular pathways involved in hypothermia's protective effects is crucial for therapeutic development.
Purpose of the Study:
- To identify and characterize microRNAs associated with the dihydropyrimidinase-like 3 (Dpysl3) gene under conditions of ischemia and hypothermia.
- To elucidate the regulatory roles of specific microRNAs in modulating Dpysl3 gene expression during ischemia-hypothermia.
Main Methods:
- PC12 cells were subjected to chemical ischemia using CoCl2 and hypothermia at 32°C.
- Exosomal microRNA analysis was performed to identify microRNAs related to the Dpysl3 gene.
- Gene expression levels were analyzed to determine the regulatory effects of identified microRNAs on Dpysl3.
Main Results:
- Four microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, and miR-497-5p) were found to be highly related to the Dpysl3 gene in ischemia-hypothermia.
- miR-497-5p demonstrated an up-regulatory effect on Dpysl3 gene expression.
- miR-106b-5p, miR-194-5p, and miR-326-5p exhibited down-regulatory effects on Dpysl3 gene expression.
Conclusions:
- The identified microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, miR-497-5p) are involved in regulating Dpysl3 gene expression under ischemia-hypothermia.
- Exosomal microRNAs represent potential therapeutic targets for managing ischemia.
- Further research into these microRNAs could lead to novel treatment strategies for ischemia.
