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Updated: Jul 9, 2025

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
Published on: March 19, 2021
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Treatment of noise caused by radiation damage during cryo-EM data collection
1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Structure (London, England : 1993)
|December 8, 2023
Abstract:
Here, we discuss how noise that is caused by radiation damage during cryo-EM data collections accumulates during single-particle analysis (SPA), MicroED, and cryo-ET. For MicroED and SPA, bad data can be identified and excluded during data collection and processing, whereas cryo-ET will require systematic radiation damage assessments that can be derived from SPA.

