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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 9, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Phage-Host Interaction Analysis Using Flow Cytometry.

Maria Daniela Silva^1,2, Luís D R Melo^3,4

¹CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.

Methods in Molecular Biology (Clifton, N.J.)

|December 8, 2023

Summary

This summary is machine-generated.

Flow cytometry offers a faster, less labor-intensive method for studying phage-host interactions compared to traditional culture methods. This technique efficiently assesses bacterial susceptibility to phage infection over time.

Keywords:

Flow cytometry Phage Synchronized infection

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Last Updated: Jul 9, 2025

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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

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Published on: April 6, 2022

Area of Science:

Microbiology
Biotechnology
Virology

Background:

Traditional culture-based methods for evaluating phage-host interactions are laborious and time-consuming.
The need for efficient, cost-effective, and less labor-intensive techniques is critical in microbiology research.
Advancements in analytical methods are crucial for understanding microbial dynamics.

Purpose of the Study:

To describe a methodology for assessing bacterial susceptibility to phage infection.
To detail the use of flow cytometry for studying phage-host interactions over time.
To present an alternative to conventional, labor-intensive culture methods.

Main Methods:

Utilizing flow cytometry to analyze planktonic bacterial cultures.
Assessing the susceptibility of bacteria to specific phage infections.
Monitoring phage-host interactions dynamically over a period.

Main Results:

Flow cytometry provides a time-efficient assessment of phage-host interactions.
The method allows for detailed study of bacterial susceptibility to phages.
This approach offers a less labor-intensive alternative to culture-based assays.

Conclusions:

Flow cytometry is a viable and efficient tool for studying phage-host dynamics.
This methodology enhances the speed and reduces the labor involved in phage-host interaction studies.
The technique facilitates a deeper understanding of bacterial responses to phage infection.