Simplified method for determination of plasma nifedipine by gas chromatography

The gas chromatographic assay for nifedipine was simplified by mixing 1 mL of plasma, 50 microL internal standard, and 950 microL of ethyl ether. After vortexing and centrifugation, the organic layer was transferred and evaporated, then reconstituted with 100 microL of ether. A 10-microL sample was injected into the GC. Several steps as well as the use of special lighting and columns are eliminated in the procedure. This method is determined to be linear, specific, sensitive, precise, and accurate.