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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.0K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Related Experiment Video

Updated: Jul 8, 2025

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

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Flow cytometry and cell sorting.

William G Telford1

  • 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

Frontiers in Medicine
|December 11, 2023
PubMed
Summary
This summary is machine-generated.

Flow cytometry cell sorting isolates cells for biomedical research. This review covers the history, mechanisms, and future of cell sorting technologies, including microfluidics.

Keywords:
cell sortingdrop delayelectrostaticflow cellflow cytometrymicrofluidicsnozzle

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Area of Science:

  • Biomedical Science
  • Cell Biology
  • Analytical Techniques

Background:

  • Flow cytometry is a key single-cell analysis tool in biomedical science.
  • Cell sorting, a technique linked to flow cytometry, enables physical separation of cells.
  • Cell isolation based on flow cytometric analysis is fundamental to understanding immune cell differentiation and development.

Purpose of the Study:

  • To review the historical development of cell sorting technology.
  • To describe the current state and multiple mechanisms of cell sorters.
  • To consider emerging cell sorting technologies, such as microfluidics.

Main Methods:

  • Historical review of cell sorting development.
  • Description of various cell sorting mechanisms.
  • Discussion of critical aspects and newer technologies in cell sorting.

Main Results:

  • Cell sorting has evolved significantly since the early days of cytometry.
  • Multiple mechanisms exist for physical cell separation based on flow cytometric data.
  • Microfluidic technologies represent a new frontier in cell sorting.

Conclusions:

  • Cell sorting is a vital technique for advancing genomic and proteomic analysis.
  • The evolution of cell sorting continues to support breakthroughs in biological research.
  • Future developments, including microfluidics, promise enhanced capabilities in cell isolation.