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Related Experiment Video

Updated: Jul 8, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP.

Andrew Leduc1, Luke Koury1, Joshua Cantlon2

  • 1Departments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, and Barnett Institute, Northeastern University, Boston, MA 02115, USA.

Biorxiv : the Preprint Server for Biology
|December 11, 2023
PubMed
Summary
This summary is machine-generated.

We developed nPOP, a novel method for parallel single-cell proteomics sample preparation. This technique enables high-throughput analysis of thousands of proteins per cell, advancing single-cell protein quantification.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Cell Biology

Background:

  • Single-cell proteomics using mass spectrometry (MS) offers high specificity and sensitivity.
  • Current methods face limitations in throughput for large-scale single-cell analysis.

Conclusions:

  • nPOP significantly enhances the throughput of single-cell proteomics.
  • The protocol is flexible, applicable to various single-cell suspensions, and facilitates broad adoption.
  • nPOP supports reliable, high-throughput single-cell protein analysis for biological discovery.