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Using eDNA for mammal inventories still needs naturalist expertise, a meta-analysis
Pauline Van Leeuwen1, Johan Michaux1
1Conservation Genetics Laboratory University of Liège Liège Belgium.
Ecology and Evolution
|December 11, 2023
Summary
Environmental DNA (eDNA) metabarcoding shows variable detection success for mammals. While effective for large mammals, it underperforms for bats compared to traditional methods, suggesting combined approaches for optimal biodiversity assessment.
Area of Science:
- Ecology
- Molecular Biology
- Conservation Biology
Background:
- Environmental DNA (eDNA) is a powerful, non-invasive tool for biodiversity assessment.
- eDNA metabarcoding is efficient in aquatic ecosystems, particularly for fish.
- Mammalian detection efficiency using eDNA metabarcoding requires further investigation.
Purpose of the Study:
- To compare the detection success of eDNA metabarcoding with conventional surveys for mammals.
- To identify factors influencing eDNA metabarcoding detection rates in mammals.
- To assess the potential of eDNA metabarcoding for comprehensive biodiversity inventories.
Main Methods:
- Meta-analysis of existing literature comparing eDNA metabarcoding and conventional survey methods.
- Inclusion of studies covering over 900 mammalian taxa.
- Analysis of detection success based on taxonomic groups and survey methods.
Main Results:
- Detection success of eDNA metabarcoding is method and taxonomy dependent.
- eDNA metabarcoding showed lower detection rates for bats compared to mist netting.
- High detection overlap between eDNA and conventional surveys was observed for large mammals (ungulates, primates, carnivores).
Conclusions:
- A combination of molecular (eDNA) and traditional field approaches enhances biodiversity assessment accuracy.
- eDNA metabarcoding has significant potential for optimization to match or exceed traditional survey efficacy.
- Further research and methodological improvements are needed to maximize eDNA metabarcoding's potential in mammalian surveys.
