Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to: "Hypoxia-Induced Lineage Plasticity in Neuroblastoma: Advancing Signature Interpretation and Clinical Translation".

Clinical and translational science·2026
Same author

Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.

Human reproduction (Oxford, England)·2026
Same author

Maternal trans-vaccenic acid shapes neonatal T cell development and early-life immune imprinting.

Science (New York, N.Y.)·2026
Same author

Spatially resolved m<sup>6</sup>A profiling using m<sup>6</sup>A-ARTR-DBiT.

Nature methods·2026
Same author

Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species.

eLife·2026
Same author

CDS-localized m<sup>6</sup>A drives co-translational RNA decay to relieve biotic and abiotic endoplasmic reticulum stresses.

Nature plants·2026

Related Experiment Video

Updated: Jul 8, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Base-Resolution Sequencing Methods for Whole-Transcriptome Quantification of mRNA Modifications.

Li-Sheng Zhang1,2,3,4, Qing Dai1,2, Chuan He1,2

  • 1Department of Chemistry, The University of Chicago, Chicago, Illinois 60637, United States.

Accounts of Chemical Research
|December 11, 2023
PubMed
Summary

New quantitative sequencing methods enable base-resolution mapping of diverse RNA modifications, including N6-methyladenosine (m6A), in mammalian mRNA. These techniques improve understanding of RNA modification stoichiometry and function.

More Related Videos

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.1K

Related Experiment Videos

Last Updated: Jul 8, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA molecules undergo chemical modifications beyond the standard bases, significantly influencing gene expression.
  • While abundant RNAs like rRNA and tRNA are heavily modified, recent research highlights functional modifications in low-abundance RNAs such as mRNA, ncRNA, and caRNA.
  • N6-methyladenosine (m6A) is the most prevalent mRNA modification in mammals, impacting mRNA processing and metabolism, typically mapped using m6A-MeRIP-seq.

Purpose of the Study:

  • To summarize recent advances in developing chemistry- or biochemistry-based methods for quantitative mapping of RNA modifications.
  • To enable base-resolution profiling and modification fraction quantification for multiple mRNA modifications in mammalian cells.
  • To facilitate a deeper understanding of RNA modification distributions, stoichiometry, and their functional implications in gene regulation and clinical applications.

Main Methods:

  • Development of novel chemistry- or biochemistry-based sequencing techniques.
  • Methods include m6A-SAC-seq, eTAM-seq, BID-seq, UBS-seq, DAMM-seq, m1A-quant-seq, Nm-Mut-seq, and m7G-quant-seq.
  • These techniques allow for base-resolution mapping and quantitative analysis of various RNA modifications with low RNA input.

Main Results:

  • Successful development of quantitative methods for mapping major mRNA modifications, including m6A, Ψ, m5C, m1A, Nm, and internal m7G, at base resolution.
  • These methods can profile diverse RNA species (mRNA, caRNA, ncRNA, mitochondrial RNA, cfRNA) with limited sample requirements.
  • The developed techniques allow for uncovering dynamic changes in modification stoichiometry during biological processes.

Conclusions:

  • The new quantitative sequencing methods offer precise mapping of multiple mRNA modifications, enhancing our understanding of their roles in gene expression.
  • These advancements facilitate investigations into RNA modifications as potential biomarkers for clinical diagnosis and prognosis.
  • The developed methods are versatile and applicable to various RNA species beyond mRNA.