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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

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ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysis.

Zülal Cibir¹, Jacqueline Hassel², Justin Sonneck^3,4

¹Institute for Experimental Immunology and Imaging, University Hospital, University of Duisburg-Essen, Essen, Germany.

Nature Communications

|December 11, 2023

Summary

This summary is machine-generated.

ComplexEye, a novel array microscope, enables high-throughput analysis of immune cell migration. This technology accelerates drug discovery and clinical applications by allowing rapid screening of compounds affecting cell movement.

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Last Updated: Jun 15, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

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Published on: May 17, 2020

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

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Published on: May 13, 2012

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Published on: December 5, 2010

Area of Science:

Cell Biology
Microscopy Technology
Immunology

Background:

Immune cell migration is crucial for health and disease.
Current methods for analyzing cell migration lack high-throughput capabilities.
Targeting cell migration has significant clinical potential.

Purpose of the Study:

To develop a high-throughput microscopy system for analyzing immune cell migration.
To enable routine migration analysis in a clinical setting.
To facilitate phenotypic screening of compounds affecting cell migration.

Main Methods:

Introduction of ComplexEye, an array microscope with 16 aberration-corrected lenses.
Utilizing 96- and 384-well plates for high-throughput (HT) cell migration assays.
High-resolution live-cell video microscopy for migration analysis.

Main Results:

ComplexEye enables energy-efficient HT migration analysis of immune cells.
The system successfully measured multiple clinical samples simultaneously.
Screened 1000 compounds, identifying 17 neutrophil migration modifiers in 4 days.

Conclusions:

ComplexEye significantly accelerates compound screening for migration modifiers.
The technology opens avenues for phenotypic HT migration screens.
Enables routine migration analysis for clinical applications.