Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ginseng-containing Shentao Ruangan (STR) formula alleviates hepatic fibrosis via hepatocellular Nrf2-ROS-TGF-β axis regulating hepatocyte-HSC crosstalk: Integrating single-cell RNA sequencing and multi-tiered validation.

Journal of ginseng research·2026

Same author

Orthogonal nanopores cross-validation for multiplex single-molecule profiling.

Chemical science·2026

Same author

In Situ Synchronized SERS-SEIRAS Unveils Cation-Regulated Interfacial Water and Intermediates in the Oxygen Reduction Reaction.

Angewandte Chemie (International ed. in English)·2026

Same author

Spatial imaging of water oxidation on single-particle catalysts.

Nature nanotechnology·2026

Same author

Targeting DNGR-1 with Fangchinoline Elevates Dendritic Cell Antigen Cross-Presentation-Mediated Antitumor Immunity in Melanoma.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Radical-Mediated Dynamic Reconstruction of Ni-N-C Single-Atom Catalysts for Wide-Potential CO<sub>2</sub>-to-CO Electroreduction.

Journal of the American Chemical Society·2026

Search research articles

Related Experiment Video

Updated: Jul 8, 2025

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Confined-Enhanced Raman Spectroscopy.

Ruiyuan Zhang¹, Lingwei Li¹, Yu Guo¹

¹Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.

|December 13, 2023

Summary

This summary is machine-generated.

Confined-enhanced Raman spectroscopy overcomes limitations of single-molecule SERS by using nanoshells to stabilize molecules on gold nanoparticles. This achieves highly sensitive, stable, and reproducible single-molecule detection for various applications.

Keywords:

Blinking signal Confined-enhanced Raman spectroscopy (CERS)In situ packaged active shell Single molecule detection Time-resolved SERS spectra

More Related Videos

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Related Experiment Videos

Last Updated: Jul 8, 2025

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Area of Science:

Analytical Chemistry
Spectroscopy
Nanotechnology

Background:

Single-molecule surface-enhanced Raman spectroscopy (SM-SERS) offers ultrahigh enhancement factors (10^14–10^15).
Commercial applications of SM-SERS are limited by challenges in achieving ultralow detection limits and signal stability.
The
on and off
blinking effect hinders reliable single-molecule analysis.

Purpose of the Study:

To develop a novel strategy to enhance Raman signal properties for ultralow detection limits.
To overcome the limitations of conventional SM-SERS, specifically signal instability and blinking.
To achieve reliable single-molecule detection with high sensitivity, stability, and reproducibility.

Main Methods:

Development of confined-enhanced Raman spectroscopy (CERS) using in situ-formed active nanoshells on plasmonic nanoparticles (gold or silver).
Utilizing the nanoshell to confine and anchor analyte molecules onto nanoparticle surfaces, preventing desorption from hot spots.
Demonstration of single-molecule detection capabilities using gold nanoparticles.

Main Results:

Remarkable improvement in overall Raman properties, including elimination of the
on and off
blinking effect.
Realization of the first single-molecule detection of analytes with super sensitivity, high stability, and reproducibility using gold nanoparticles.
Validation of the strategy's suitability for diverse molecule systems.

Conclusions:

Confined-enhanced Raman spectroscopy provides a robust platform for stable and sensitive single-molecule analysis.
The developed nanoshell strategy significantly advances SM-SERS capabilities for practical applications.
This technique shows promise for applications in biomedical diagnosis, catalytic reactions, and other fields requiring high-sensitivity molecular detection.