Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse Southern hybridization.

H B Gamper, G D Cimino, S T Isaacs

    Nucleic Acids Research
    |December 22, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Report from the 20th annual Western Canadian Gastrointestinal Cancer Consensus Conference; Saskatoon, Saskatchewan; 28-29 September 2018.

    Current oncology (Toronto, Ont.)·2020
    Same author

    A Data Processing Pipeline for Prediction of Milling Machine Tool Condition from Raw Sensor Data.

    Smart and sustainable manufacturing systems·2019
    Same author

    Cancer league actions against sunbed use for skin cancer prevention.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2019
    Same author

    eHealth readiness of dietitians.

    Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2018
    Same author

    Routine germline BRCA1 and BRCA2 testing in patients with ovarian carcinoma: analysis of the Scottish real-life experience.

    BJOG : an international journal of obstetrics and gynaecology·2018
    Same author

    Feasibility of a novel participatory multi-sector continuous improvement approach to enhance food security in remote Indigenous Australian communities.

    SSM - population health·2018

    This study introduces a novel DNA probe using a 4'-hydroxymethyl-4,5', 8-trimethylpsoralen (HMT) monoadduct. This probe enables rapid and sensitive detection of specific DNA sequences in M13 mp19 and pUC 19 DNA.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • DNA hybridization is crucial for molecular biology techniques.
    • Photocrosslinking agents can enhance probe-target complex stability.
    • Developing sensitive DNA detection methods is essential for genetic analysis.

    Purpose of the Study:

    • To develop a sensitive DNA detection probe using a modified oligomer.
    • To investigate the efficacy of simultaneous hybridization and photofixation.
    • To assess the probe's detection limit for specific DNA sequences.

    Main Methods:

    • Synthesis of a 25-nucleotide DNA oligomer with an HMT monoadduct.
    • Simultaneous hybridization and photofixation in solution.
    • Centrifugation for probe removal and alkaline agarose gel electrophoresis.

    Related Experiment Videos

  • Autoradiography for signal detection.

    • Main Results:

    • The HMT-modified probe achieved hybridization and photofixation in minutes.
    • Near-UV irradiation efficiently converted monoadducts to interstrand crosslinks.
    • Detection of as little as 0.015 ng of M13 DNA was achieved.
    • No signal was detected for 10 micrograms of denatured human DNA, indicating specificity.

    Conclusions:

    • The HMT-modified DNA probe offers a rapid and highly sensitive method for detecting specific DNA sequences.
    • Simultaneous hybridization and photofixation significantly enhances probe-target complex stability.
    • This technique demonstrates high specificity, distinguishing target DNA from complex genomic backgrounds.