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Updated: Jul 2, 2026
Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees
Published on: October 15, 2021
In situ chlorpyrifos (CPF) degradation by Acrobeloides maximus: Insights from chromatographic analysis
Shweta P Thakar1, Ranjitsinh C Dabhi2, Suryajit L Rathod2
1Department of Zoology, Biomedical Technology, Human Genetics and Wildlife Conservation and Biology, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad, Gujarat 380009, India.
Abstract:
The objective of this study was to evaluate the efficiency of nematodes in zooremediation of chlorpyrifos (CPF), an organophosphate pesticide. The nematode population Acrobeloides maximus (A. maximus) was employed for bioremediation, converting CPF into non-toxic residues. Optimal growth conditions for mass production of A. maximus were achieved by maintaining a temperature of 25 °C, pH 8, and supplementing the culture medium with plant nutrients. The nematodes were then immobilized within sodium alginate beads. The efficacy of the degradation process was assessed using various analytical techniques, including UV-Visible spectroscopy, HPTLC, FTIR, and LC-MS, confirming the successful breakdown of CPF. The bioreactor demonstrated a complete degradation efficiency of CPF exceeding 99%. Additionally, LC-MS analysis was conducted to elucidate the degradation pathway based on the formation of intermediates. These results underscore the potential of A. maximus as a sustainable organism for addressing environmental contamination arising from CPF pesticide.
