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Updated: Aug 19, 2026

17:03
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
Published on: March 23, 2010
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Dynamic monitoring of an enzymatically driven dissipative toehold-mediated strand displacement reaction
Shuang Li1, Disong Zhao1, Fangfang Yang1
1College of Chemistry and Chemical Engineering, Yantai University, 30 Qingquan Road, Yantai 264005, China. yangfangfang@ytu.edu.cn.
Abstract:
A general strategy to program self-resettable and dissipative toehold-mediated strand displacement reactions was proposed, using DNA strands as the fuel and lambda exonuclease as the fuel-consuming unit. This non-equilibrium system is reversible and temporally controllable. Furthermore, it can be well integrated into a DNA network to temporally control its cascade reaction or dynamic behaviour.

