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Electrotransfer for nucleic acid and protein delivery.

Aswin Muralidharan1, Pouyan E Boukany2

  • 1Department of Bionanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands.

Trends in Biotechnology
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Summary

Electrotransfer is vital for gene therapy and editing, enabling delivery of nucleic acids and proteins into cells. Emerging technologies enhance its biomedical applications, including clinical trials for immune and stem cell therapies.

Keywords:
cell engineeringelectroporationelectrotransfergene deliverynon-viral gene delivery

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Electrotransfer is a key method for introducing nucleic acids and proteins into cells.
  • It is essential for gene augmentation and genome editing applications.
  • Biomedical applications are rapidly expanding.

Purpose of the Study:

  • To review the applications of electrotransfer in biotechnology and medicine.
  • To highlight successful clinical trials and therapeutic cell targeting.
  • To explore advancements in electrotransfer technologies.

Main Methods:

  • Review of existing literature on electrotransfer.
  • Analysis of clinical trial data.
  • Exploration of emerging technologies like nanotechnology and deep learning.

Main Results:

  • Electrotransfer is successfully used ex vivo and in vivo for therapeutic purposes.
  • Nucleic acid and protein delivery into immune cells, stem cells, and progenitor cells has been achieved.
  • Nanotechnology and deep learning show promise in overcoming electroporation limitations.

Conclusions:

  • Electrotransfer is a powerful tool with significant current and future biomedical potential.
  • Advancements in nanotechnology and deep learning are expanding the scope of electrotransfer applications.
  • Further research into these emerging areas will drive innovation in gene and protein therapies.