Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hard-to-detect mutations explain how common autoimmune diseases arise.

Nature·2026
Same author

LIF-Induced Tumor Plasticity Establishes an Immunosuppressive Myeloid Niche in LKB1-Mutant Lung Cancer.

Cancer discovery·2026
Same author

Pharmacological reduction of neutrophil infiltration reduces <i>Clostridioides difficile</i> infection severity.

mBio·2026
Same author

Functional divergence of the gut microbiome associated with lifestyle and helminth infection in Indigenous Peninsular Malaysian.

Research square·2026
Same author

The integrated stress response promotes immune evasion through lipocalin 2.

Nature·2026
Same author

A STAT3 degrader demonstrates efficacy in venetoclax resistant acute myeloid leukemia.

Leukemia·2026

Related Experiment Video

Updated: Jul 8, 2025

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.2K

Temporal Tracking of Plasma Cells in vivo Using J-chain CreERT2 Reporter System.

Timothy C Borbet1, Kimberly Zaldaña1, Anastasia-Maria Zavitsanou1,2

  • 1Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.

Biorxiv : the Preprint Server for Biology
|December 18, 2023
PubMed
Summary

Researchers developed a new mouse model, IgJCreERT2, to track plasma cells (PCs) and study antibody production. This tool helps differentiate long-lived and short-lived PCs, advancing research in humoral immunity and vaccine responses.

More Related Videos

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

8.9K
Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells
10:19

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

1.3K

Related Experiment Videos

Last Updated: Jul 8, 2025

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.2K
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
11:34

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

8.9K
Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells
10:19

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

1.3K

Area of Science:

  • Immunology
  • Genetics

Background:

  • Plasma cells (PCs) are crucial for antibody production and immunological memory.
  • Distinguishing between long-lived and short-lived PCs in vivo is challenging due to a lack of specific markers.

Approach:

  • Developed a novel J-chain CreERT2 GFP allele (IgJCreERT2) mouse model for temporal and cell-specific PC tracking.
  • Utilized tamoxifen-inducible Cre recombinase based on PC-restricted J-chain gene expression.
  • Validated the model in vitro and in vivo for tracing long-lived PCs after immunization.

Key Points:

  • The IgJCreERT2 model accurately traces long-lived plasma cells in vivo.
  • Enabled detailed analysis of PC surface markers, revealing heterogeneity.
  • Confirmed the model's utility for studying PC dynamics and longevity.

Conclusions:

  • The IgJCreERT2 mouse is a reliable tool for in-depth plasma cell research.
  • Facilitates investigation of PC dynamics in humoral immunity and vaccine responses.
  • Offers new avenues for exploring plasma cell biology and immunological memory in health and disease.