Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
X-ray Crystallography02:18

X-ray Crystallography

23.9K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
23.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.

bioRxiv : the preprint server for biology·2026
Same author

Mechanical movements generated by movable lipids break endosomal barriers for enhanced mRNA therapeutics.

Science advances·2026
Same author

A novel translation initiation codon single nucleotide variant of the SLC14A1 (c.2T>G) from a Chinese blood donor with Jk(a-b-) phenotype.

Transfusion·2026
Same author

A pico-calorimeter for cellular metabolism and antimicrobial susceptibility testing.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics.

Optics express·2026
Same author

Single-Cell-Derived Rabbit mAb-Based Immunoassay for Simultaneous Detection of Florfenicol and Florfenicol Amine.

Analytical chemistry·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jul 8, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.2K

Multi-harmonic structured illumination-based optical diffraction tomography.

Ruihua Liu, Kai Wen, Jiaoyue Li

    Applied Optics
    |December 18, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces multi-harmonic structured illumination-based optical diffraction tomography (MHSI-ODT) for faster 3D refractive index imaging. MHSI-ODT improves imaging speed by 16.7% and enhances intensity utilization for transparent samples.

    More Related Videos

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.1K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.9K

    Related Experiment Videos

    Last Updated: Jul 8, 2025

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.2K
    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.1K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.9K

    Area of Science:

    • Optical imaging
    • Tomography
    • Biophysics

    Background:

    • 3D refractive index (RI) imaging faces a trade-off between imaging speed and spatial resolution.
    • Conventional optical diffraction tomography (ODT) methods have limitations in achieving both high speed and resolution.

    Purpose of the Study:

    • To present a novel multi-harmonic structured illumination-based optical diffraction tomography (MHSI-ODT) technique.
    • To enhance imaging speed and intensity utilization in 3D RI mapping of transparent samples.

    Main Methods:

    • Utilized a digital micromirror device (DMD) to generate multi-harmonic structured illumination.
    • Acquired 3D RI maps by solving spectral crowns from phase-shifted holograms.
    • Employed both 1st-order and 2nd-order harmonics for improved illumination intensity utilization.

    Main Results:

    • MHSI-ODT enhances imaging speed by 16.7% compared to conventional SI-ODT.
    • Demonstrated the capability to render 3D RI distributions of polystyrene microspheres and biological samples.
    • Achieved better intensity utilization of structured illumination.

    Conclusions:

    • MHSI-ODT offers a significant advancement in 3D refractive index imaging speed and efficiency.
    • The technique is effective for imaging various transparent samples, including microparticles and biological specimens.