Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered crRNA for CRISPR/Cas-assisted biosensing.

Trends in biotechnology·2024
Same author

Ecological drivers of nesting behavior in a subtropical city: An observational study on spotted doves.

Ecology and evolution·2024
Same author

Inulin enhanced rifaximin-inhibited colon cancer pulmonary metastasis by flora-regulated bile acid pathway.

International journal of biological macromolecules·2024
Same author

The T-cell receptor β chain CDR3 insights of bovine liver immune repertoire under heat stress.

Animal bioscience·2024
Same author

Computing Smooth and Integrable Cross Fields via Iterative Singularity Adjustment.

IEEE transactions on visualization and computer graphics·2024
Same author

Comparison of short-segment and long-segment fixation in treatment of degenerative scoliosis and analysis of factors associated with adjacent spondylolisthesis.

Open medicine (Warsaw, Poland)·2024

Related Experiment Video

Updated: Jul 8, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

16.7K

Robust point light source calibration method for near-field photometric stereo using feature points selection.

Long Ma, Xu Liu, Yuzhe Liu

    Applied Optics
    |December 18, 2023
    PubMed
    Summary

    This study introduces a robust method for calibrating non-isotropic point light sources using feature point selection and an l_p-norm cost function. The approach ensures accurate light source parameter estimation even with noisy image data.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.8K
    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.5K

    Related Experiment Videos

    Last Updated: Jul 8, 2025

    Determining 3D Flow Fields via Multi-camera Light Field Imaging
    14:25

    Determining 3D Flow Fields via Multi-camera Light Field Imaging

    Published on: March 6, 2013

    16.7K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.8K
    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.5K

    Area of Science:

    • Computer Vision
    • Optical Engineering
    • Photometry

    Background:

    • Accurate calibration of non-isotropic point light sources is crucial for photometric stereo and 3D reconstruction.
    • Existing methods often struggle with noisy observations and efficiency.

    Purpose of the Study:

    • To develop a robust and efficient method for non-isotropic point light source calibration.
    • To improve the accuracy of light source parameter estimation in the presence of noise.

    Main Methods:

    • A novel feature point selection method analyzing surface-image intensity relationships under near-field lighting.
    • Establishment of an l_p-norm cost function for calibration.
    • Application of an improved Newton method-based iteration for parameter calculation.

    Main Results:

    • Robust calibration achieved with estimation errors below 2.7 mm and 0.8°.
    • Method effective even with Gaussian white noise (standard deviation up to 0.4).
    • Experimental validation using a photometric stereo system on standard sphere and compressor blade.

    Conclusions:

    • The proposed method offers a robust and accurate solution for non-isotropic point light source calibration.
    • Demonstrated effectiveness in real-world applications with noisy image data.