Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

50.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
Homologous Recombination02:31

Homologous Recombination

50.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B7-H3 CAR T cells eradicate intrahepatic cholangiocarcinoma and induce durable response.

Journal of experimental & clinical cancer research : CR·2026
Same author

[Corrigendum] Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA‑140‑3p/SMAD2 signaling pathway in idiopathic short stature.

International journal of molecular medicine·2026
Same author

Capsaicin diet drives gut inflammation and exosomal miR-17-3p elevation in idiopathic short stature.

Nature communications·2025
Same author

Targeting MT-tsRNA IDDMT with Nanoparticles to Annulus Fibrosus: A Novel Therapeutic Approach for Intervertebral Disc Degeneration.

Advanced healthcare materials·2025
Same author

Simultaneous Recovery of Magnesium and Lithium from Salt Lake Brine by Membrane Electrolysis for Resource Utilization.

Materials (Basel, Switzerland)·2025
Same author

Unveiling the temporal and spatial trajectories of early resistance formation during Hylocereus undatus senescence through single-cell transcriptomics.

Plant molecular biology·2025
Same journal

Make uphill thermodynamics downhill in pathway design.

Trends in biotechnology·2026
Same journal

Engineering a capture-bioremediate-release microbial biofilm for simultaneous bioremediation of microplastics and adsorbed heavy metals.

Trends in biotechnology·2026
Same journal

Engineered bacterial biofilms for biotechnological applications.

Trends in biotechnology·2026
Same journal

Multiscale and programmable engineering of edible mushroom mycelium-based materials.

Trends in biotechnology·2026
Same journal

Transporter engineering in microbial cell factories.

Trends in biotechnology·2026
Same journal

Random integration and high-throughput screening forging robust microbial cell factories.

Trends in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K

Engineered crRNA for CRISPR/Cas-assisted biosensing.

Long Ma1, Minghui Lu1, Jingyu Jia1

  • 1Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin, 300457, China; Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin, 300457, China; China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin, 300457, China.

Trends in Biotechnology
|July 9, 2024
PubMed
Summary
This summary is machine-generated.

Engineered CRISPR RNA (crRNA) advances overcome limitations in CRISPR-based diagnostics (CRISPR-Dx). These innovations improve detection of short nucleotides and non-nucleic acid targets, enhancing diagnostic capabilities.

Keywords:
CRISPR/Caschallenges and opportunitiesdetection, CRISPR-Dxengineered crRNA

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

215

Related Experiment Videos

Last Updated: Jun 21, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

215

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Diagnostics

Background:

  • CRISPR/Cas-based diagnostics (CRISPR-Dx) offer promising molecular detection but face significant challenges.
  • Current limitations include poor detection of ultrashort nucleotides, reliance on preamplification, cross-contamination risks, and difficulties with on-pot and non-nucleic acid targets.

Purpose of the Study:

  • To review recent advancements in engineered CRISPR RNA (crRNA) design for CRISPR-Dx.
  • To highlight how these engineered crRNAs address existing challenges in CRISPR-based diagnostics.

Main Methods:

  • Review of literature on engineered CRISPR RNA (crRNA) modifications and applications in CRISPR-based diagnostics.
  • Analysis of strategies to overcome limitations in sensitivity, specificity, and target range.

Main Results:

  • Engineered crRNAs demonstrate enhanced capabilities in detecting ultrashort nucleotides.
  • New designs mitigate preamplification dependency and reduce cross-contamination risks.
  • Progress has been made in developing on-pot detection and expanding target detection to non-nucleic acid molecules.

Conclusions:

  • Engineered CRISPR RNA (crRNA) represents a significant step forward in overcoming CRISPR-Dx limitations.
  • These advancements pave the way for more robust, versatile, and user-friendly CRISPR-based diagnostic tools.
  • Future research should focus on further optimizing crRNA designs and integrating them into point-of-care applications.