[Survivability and penetration capacity of bull spermatozoa frozen by 3 technics]

Studied was the survival and penetration capacity of bull spermatozoa frozen after the following technologies--pellet, straw, and minitube--and the results obtained were compared via biologic experiments. The minitube technology of freezing the semen led to higher thermal resistance of the spermatozoa as against freezing in the form of straws and pallets (363.5 +/- 8.02 min, 356.25 +/- 7.79 min, and 339.00 +/- 8.44 min, respectively). The differences established were statistically highly significant (P less than 0.001). The penetration capacity of spermatozoa in an estral secretion of cows was highest at freezing in the form of minitubes (1.70 +/- 0.54 mm/min). The same was lower with straws (1.53 +/- 0.02 mm/min), and lowest--with pellets (1.52 +/- 0.04 mm/min). The differences were statistically significant (P less than 0.01). The fertilization capacity of spermatozoa frozen with the employment of the three technologies was best with the use of minitubes and straws--50.20, resp., 49.67 per cent, and lowest with the use of pellets--45.22 per cent.