[A modified highly sensitive spectrophotometric method for determining acid phosphatase activity in the animal liver]

A modified spectrophotometric procedure is described for estimation of acid phosphatase activity in rabbit liver tissue. Coefficient of instrumental sensitivity (SA/C = 0.029), apparent molar absorption (epsilon 820 = 30,500), relative sensitivity of measurement (Cmin = 0.18 mM) were calculated as well as main metrological patterns were evaluated, which showed that the procedure was highly sensitive, accurate and reproducible. Standard error did not exceed 1% and standard deviation was not above 5%.