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A simple fluorescence pyrocatechol-polyethyleneimine detection method for 3-MCPD.

Ting Xu1, Qing Fu1, Zeng Qingru1

  • 1Institute of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. fangmin0227@whpu.edu.cn.

Analytical Methods : Advancing Methods and Applications
|December 19, 2023
PubMed
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A new fluorescence method rapidly detects 3-monochloropropane-1,2-diol (3-MCPD) using pyrocatechol-polyethyleneimine polymer. This sensitive technique offers quick 3-MCPD contamination analysis in food products.

Area of Science:

  • Analytical Chemistry
  • Food Science
  • Polymer Science

Background:

  • 3-monochloropropane-1,2-diol (3-MCPD) is a food contaminant requiring sensitive detection methods.
  • Existing detection techniques for 3-MCPD can be time-consuming and complex.
  • Development of rapid, sensitive, and cost-effective analytical methods is crucial for food safety.

Purpose of the Study:

  • To establish a rapid fluorescence detection method for 3-MCPD.
  • To investigate the fluorescence quenching mechanism of 3-MCPD on a pyrocatechol-polyethyleneimine (PCh-PEI) polymer.
  • To optimize experimental conditions for sensitive and accurate 3-MCPD quantification.

Main Methods:

  • Utilized a pyrocatechol-polyethyleneimine (PCh-PEI) polymer as a fluorescent probe.

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  • Investigated the fluorescence quenching effect of 3-MCPD on PCh-PEI under varying pH and temperature conditions.
  • Determined the optimal conditions for detection (pH 8.5 and 100 °C).
  • Examined the effect of different 3-MCPD concentrations on the polymer's optical properties.

    • Main Results:

    • The PCh-PEI polymer exhibited fluorescence quenching in the presence of 3-MCPD.
    • Optimal quenching efficiency was achieved at pH 8.5 and 100 °C.
    • The fluorescence detection method showed linearity in the range of 0.08–2.0 mg L⁻¹ 3-MCPD.
    • A low detection limit of 0.06 mg L⁻¹ and a high correlation coefficient (0.9974) were achieved.

    Conclusions:

    • A sensitive and rapid fluorescence detection method for 3-MCPD has been successfully developed.
    • The PCh-PEI polymer serves as an effective fluorescent probe for 3-MCPD detection.
    • The method is suitable for the rapid screening of 3-MCPD contamination in food products, enhancing food safety monitoring.