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Updated: Jul 8, 2025

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Published on: February 27, 2016

Toward building comprehensive particle tracking tools with u-track 3D.

Lance W Q Xu¹, Steve Pressé²

¹Center for Biological Physics, Arizona State University, Tempe, AZ 85287, USA; Department of Physics, Arizona State University, Tempe, AZ 85287, USA.

Cell Reports Methods

|December 19, 2023

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Roudot et al. introduce u-track 3D, a new software package for offline widefield multi-molecule tracking. This tool enhances visualization, tracking, and trackability assessment for biological particle analysis.

Area of Science:

Biophysics
Cell Biology
Computational Biology

Background:

Accurate tracking of molecules in biological systems is crucial for understanding cellular processes.
Existing methods for offline widefield multi-molecule tracking can be complex and time-consuming.
There is a need for improved tools to streamline the analysis of particle movement in biological samples.

Purpose of the Study:

To present u-track 3D, a novel software package designed to enhance the workflow of offline widefield multi-molecule tracking.
To provide researchers with an integrated tool for visualization, tracking, and assessment of trackability in biological particle tracking.
To facilitate more efficient and accurate analysis of dynamic biological processes at the molecular level.

Main Methods:

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Related Experiment Videos

Last Updated: Jul 8, 2025

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Published on: February 27, 2016

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Published on: July 20, 2017

Development of the u-track 3D software package.

Implementation of algorithms for particle tracking in widefield microscopy images.

Inclusion of features for visualization of particle trajectories and assessment of tracking quality.

Main Results:

u-track 3D offers an improved workflow for offline widefield multi-molecule tracking.
The package enables effective visualization of particle tracks.
It provides tools for robust tracking and assessment of trackability in complex biological systems.

Conclusions:

u-track 3D is a valuable tool for researchers studying molecular dynamics in biological systems.
The software package simplifies and enhances the analysis of particle tracking data.
This contributes to advancing the understanding of cellular mechanisms through improved imaging analysis.