Surveillance of SARS-CoV-2 RNA in wastewater matrix: a review

SARS-CoV-2 is the agent responsible for the global pandemic sickness, COVID-19. It is an enveloped virus that belongs to the family Coronaviridae. Recent studies have revealed the fecal shedding of the virus and have been found to enter wastewater and aquatic systems. Prolonged viral presence in fecal samples is a common observation in the reported literature. Survival of the virus in the recipient environment could be a crucial factor that influences its fecal-oral transmission. The detection of a novel coronavirus in wastewater opportunity has potential for environmental surveillance at the community or population level. Such a surveillance system can enable the early detection of disease outbreaks in zones with pre-symptomatic/asymptomatic patients and act as a complementary tool for continuous monitoring of quarantine zones. In contrast to developed regions, resource constraints in underdeveloped communities coupled with different sanitation settings may pose a challenge to wastewater sampling and surveillance. To begin, this review summarizes the literature on the presence of SARS-CoV-2 in feces. The approaches for viral extraction, concentration, and detection in wastewater matrices are then highlighted. Finally, investigations on wastewater-based epidemiology for SARS-CoV-2 surveillance are reviewed.