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Updated: Jul 7, 2025

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

Bioengineered Tubular Biliary Organoids.

Bilge Sen Elci¹, Mikhail Nikolaev^1,2, Saba Rezakhani^1,2

¹Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland.

Advanced Healthcare Materials

|December 21, 2023

Summary

This summary is machine-generated.

This study introduces a novel hydrogel organoid-on-a-chip system for creating functional tubular biliary organoids. This advanced model offers long-term stability and better mimics the human biliary tree for drug discovery and disease research.

Keywords:

bile duct bioengineering liver ductal organoids organ‐on‐a‐chip

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Last Updated: Jul 7, 2025

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

Published on: April 16, 2021

Area of Science:

Biotechnology
Regenerative Medicine
Organoid Technology

Background:

Conventional liver organoids have limitations in culture duration and physiological relevance.
Existing 3D cystic organoid models lack accessible apical surfaces and in vivo-like organization.

Purpose of the Study:

To develop a hydrogel-based organoid-on-a-chip model for functional tubular biliary organoids.
To overcome limitations of conventional organoid culture systems for improved in vitro modeling.

Main Methods:

Utilized a hydrogel-based organoid-on-a-chip system.
Induced in vitro formation of a perfusable branching biliary network.
Co-cultured biliary organoids with vascular endothelial cells and fibroblasts.

Main Results:

Achieved long-term stability of biliary organoids for over 45 days.
Demonstrated retention of biliary organoid identity and cholangiocyte characteristics.
Successfully mimicked the intrahepatic biliary tree structure and enhanced cellular complexity.

Conclusions:

The developed tubular biliary organoid model offers enhanced stability and physiological relevance.
This model provides a superior platform for studying biliary morphogenesis and pathophysiology.
The organoid-on-a-chip system advances in vitro toxicology, drug discovery, and disease modeling.