Evaluation of AgNCs@PEI and their integrated hydrogel for colorimetric and fluorometric detection of ascorbic acid

A dual-mode colorimetric and fluorometric sensor based on water soluble silver nanoclusters (AgNCs@PEI) is developed for quantitative and visual detection of ascorbic acid (Asc A). The detection method relies on the Asc A induced aggregation of AgNCs@PEI, which resulted in fluorecsence quenching of the sensor. The clusters exhibited a unique combination of static and collisional quenching with a wide range of dynamic detection (1-10⁵ µM) Linear relationship was observed in the concentration range 10²-10³ µM using fluorescence and 0.2 × 10²-5 × 10³ microM using absorbance spectroscopy with respective detection limits of 10.65 microM and 2.49 microM. The corresponding colorimetric and fluorometric changes can be easily monitored by the naked eye with a visual detection limit of 10³ microM. AgNCs@PEI were further integrated within a hydrogel for developing a solid-state visual detection platform. Notably, the sensing response of the clusters towards Asc A remained unaltered even after hydrogel integration. Additionally, digital image analysis was adopted, which improved the sensitivity of instrument-free fluorescence detection of Asc A. Analysis by the developed sensor showed excellent recovery percentages of Asc A in spiked urine samples, which further underscores the practical applicability of the sensor.