Related Experiment Video
Updated: Jul 7, 2025
An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
Published on: July 13, 2009
Application of an Antioxidant Response Element-Nuclear Factor Erythroid 2 Luciferase Assay for Assessing the Skin
Ji-Yeon Yang1, Soo-Jin Park1, Ji-Young Shin1
1Toxicity and Risk Assessment Division, Department of Agro-Food Safety & Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Jeollabuk-do, Republic of Korea.
Abstract:
The skin sensitization potential of agrochemicals can be assessed using laboratory methods such as the keratinocyte activation assay so that their use in regulatory toxicology might replace experimental animal testing. Here, we evaluated the skin sensitization potential of 11 agrochemicals by using an antioxidant response element-nuclear factor erythroid 2 luciferase assay in KeratinoSens and LuSens cells and applying a skin sensitization adverse outcome pathway (AOP). The KeratinoSens and LuSens assays consistently evaluated the skin sensitization potential of 10/11 agrochemicals with reference to animal testing databases. Benomyl, pretilachlor, fluazinam, terbufos, butachlor, and carbosulfan were correctly detected as sensitizers, and glufosinate ammonium, oxiadiazon, tebuconazole, and etofenprox were correctly detected as non-sensitizers. For diazinon, the skin sensitizing potential was positive in the KeratinoSens assay but not in the LuSens assay. These results suggest that the evaluation of in vitro skin sensitization using the AOP mechanism can be applied to assess active agrochemicals.
