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Development and validation of a UPLC-PDA method for quantifying ceftazidime in dried blood spots
Jianmei Lv1, Qiping Wu1, Sanwang Li2
1Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
Journal of Pharmaceutical and Biomedical Analysis
|December 22, 2023
Summary
This study developed a new method using dried blood spots (DBS) to measure ceftazidime levels in newborns. This technique supports crucial pharmacokinetic studies for treating neonatal bacterial infections.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Neonatal Medicine
Background:
- Bacterial infections are a major cause of neonatal mortality.
- Ceftazidime is vital for treating neonatal infections, but its use is often off-label.
- Traditional blood sampling for pharmacokinetic studies in neonates is challenging.
Purpose of the Study:
- To develop and validate an ultra-performance liquid chromatography with a photodiode array (UPLC-PDA) method.
- To quantify ceftazidime in dried blood spots (DBS) from neonatal patients.
- To support pharmacokinetic studies for neonatal infections.
Main Methods:
- Utilized Capitainer® device for 10 µL DBS collection.
- Extracted ceftazidime using 70% methanol/water with acetaminophen as internal standard.
- Employed an Acquity UPLC HSS T3 column with a 3.2-minute run time and PDA detection at 259 nm.
Main Results:
- The UPLC-PDA method demonstrated high accuracy (90.1–104.8%) and precision (4.8–11.7% CV).
- The quantification range for ceftazidime was 0.5–200 µg/mL.
- No interfering peaks were observed, confirming method specificity.
Conclusions:
- A validated UPLC-PDA method for ceftazidime in neonatal DBS was successfully developed.
- This microsampling technique facilitates pharmacokinetic studies in neonates, aiding treatment optimization.
- The method is applicable to clinical samples from neonatal patients.
