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Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

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Methods for Engineering Binders to Multi-Pass Membrane Proteins.

Benjamin Thomas1, Karuppiah Chockalingam2, Zhilei Chen1,2

  • 1Interdisciplinary Graduate Program in Genetics and Genomics, Texas A&M University, College Station, TX 77845, USA.

Bioengineering (Basel, Switzerland)
|December 23, 2023
PubMed
Summary
This summary is machine-generated.

Engineering biologics against multi-pass membrane proteins is challenging. This review covers methods for preparing these proteins to develop specific therapeutics and research tools.

Keywords:
SMALPdirected evolutionmulti-pass transmembrane proteinsnanodiscpanningtransmembrane proteins

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Multi-pass membrane proteins (MMPs) are crucial cell surface drug targets.
  • Engineering biologics against MMPs presents significant technical hurdles.
  • Existing methods for MMPs present limitations for biologic development.

Purpose of the Study:

  • To review methods for preparing and presenting MMPs for biologic engineering.
  • To highlight advancements in developing target-specific biologics like antibodies and nanobodies.
  • To discuss applications of engineered biologics in therapeutics and research.

Main Methods:

  • Focus on publications from the last 10 years.
  • Analysis of different formats for presenting MMPs.
  • Review of techniques for engineering antibodies, nanobodies, and scaffold proteins.

Main Results:

  • Engineered biologics demonstrate high specificity and affinity for MMP targets.
  • Successful applications include therapeutics, cell staining probes, and crystallization chaperones.
  • New technologies are emerging to address existing challenges.

Conclusions:

  • Progress in preparing MMPs facilitates the development of novel biologics.
  • Engineered biologics offer promising solutions for therapeutic and research applications.
  • Continued innovation is key to overcoming remaining challenges in MMP targeting.