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BLV-miR-B1-5p Promotes Staphylococcus aureus Adhesion to Mammary Epithelial Cells by Targeting MUC1

Shuai Lian1,2,3, Pengfei Liu1,2,3, Xiao Li1,2,3

  • 1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Animals : an Open Access Journal From MDPI
|December 23, 2023
PubMed

Insights

Bovine leukemia virus microRNA B1-5p (BLV-miR-B1-5p) enhances Staphylococcus aureus adhesion to cow mammary cells. It achieves this by targeting and inhibiting MUC1 expression, a key factor in cellular defense.

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Dairy Science

Background:

  • Bovine leukemia virus (BLV) infection is globally prevalent in dairy cows, impairing mammary epithelial cell defenses.
  • BLV-encoded microRNAs (BLV-miRNAs) are implicated in modulating host gene expression and facilitating viral replication.
  • Previous research indicated BLV-miR-B1-5p promotes Staphylococcus aureus (S. aureus) adhesion to bovine mammary epithelial (MAC-T) cells, but the mechanism was unknown.

Purpose of the Study:

  • To elucidate the molecular pathway through which BLV-miR-B1-5p enhances S. aureus adhesion to MAC-T cells.
  • To identify and validate the target gene of BLV-miR-B1-5p involved in S. aureus adhesion.
  • To understand the role of MUC1 in BLV-miR-B1-5p-mediated S. aureus adhesion.

Main Methods:

  • Bioinformatic prediction of BLV-miR-B1-5p target genes.
  • Validation using real-time polymerase chain reaction (RT-PCR) to assess gene expression.
  • Immunofluorescence assays to visualize protein localization and expression.
  • Dual luciferase reporter assays to confirm direct targeting and inhibition of gene expression.

Main Results:

  • BLV-miR-B1-5p was predicted to target the mucin 1 (MUC1) gene.
  • Experimental validation confirmed that BLV-miR-B1-5p directly targets and inhibits MUC1 expression in MAC-T cells.
  • The inhibition of MUC1 by BLV-miR-B1-5p was shown to promote S. aureus adhesion to MAC-T cells.

Conclusions:

  • BLV-miR-B1-5p promotes S. aureus adhesion to bovine mammary epithelial cells.
  • This promotion occurs through the specific targeting and downregulation of the MUC1 gene.
  • Understanding this mechanism provides insights into BLV pathogenesis and potential strategies for controlling S. aureus mastitis in dairy cattle.
Keywords:
BLVBLV-miR-B1-5pMUC1Staphylococcus aureusdairy cows

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