SNPs-Panel Polymorphism Variations in GHRL and GHSR Genes Are Not Associated with Prostate Cancer

Area of Science:

• Genetics
• Oncology
• Molecular Biology

Background:

• Prostate cancer (PCa) is a global health concern with complex etiological factors.
• Ghrelin and its receptor (GHSR) have been implicated in various cancers, including PCa.
• Previous studies on ghrelin (GHRL) and GHSR gene polymorphisms and PCa susceptibility have yielded conflicting results.

Purpose of the Study:

• To investigate the association between specific single nucleotide polymorphisms (SNPs) in the GHRL and GHSR genes and sporadic PCa susceptibility.
• To explore potential gene-gene interactions among these SNPs in relation to PCa risk.

Main Methods:

• A case-control study involving 120 PCa patients and 95 healthy controls.
• Genotyping of six tag SNPs (three in GHRL: rs696217, rs4684677, rs3491141; three in GHSR: rs2922126, rs572169, rs2948694) using TaqMan assays.
• Analysis of allele, genotype, haplotype frequencies, linkage disequilibrium (LD), and gene-gene interactions using multifactor dimensionality reduction (MDR).

Main Results:

• No significant association was found for most GHRL and GHSR polymorphisms with PCa susceptibility (p > 0.05).
• The GHSR rs2922126 TT genotype was significantly more frequent in PCa patients (p = 0.040), suggesting it as a potential susceptibility genotype.
• MDR analysis identified a significant gene-gene interaction between GHSR rs2922126 and rs572169 (81.08% accuracy, p = 0.0001) for PCa susceptibility prediction.

Conclusions:

• While most individual GHRL and GHSR polymorphisms are not strongly associated with PCa, the GHSR rs2922126 TT genotype may confer susceptibility.
• Significant gene-gene interactions between GHSR rs2922126 and rs572169 play a role in PCa susceptibility.
• These findings offer insights into the genetic underpinnings of PCa and may inform clinical strategies for prevention and management.