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Related Experiment Video

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Assessing Freshwater Microbiomes from Different Storage Sources in the Caribbean Using DNA Metabarcoding.

Joseph Cross1,2, Prasanna Honnavar3, Xegfred Lou T Quidet4

  • 1Department of Biochemistry, Cell Biology and Genetics, American University of Antigua College of Medicine, St. Johns 1451, Antigua and Barbuda.

Microorganisms
|December 23, 2023
PubMed
Summary

DNA metabarcoding offers a superior method for assessing drinking water quality, revealing distinct bacterial microbiomes in various freshwater sources. This technique effectively identifies potentially pathogenic species, enhancing public health surveillance in regions like Antigua and Barbuda.

Keywords:
NGSmetabarcodingmicrobiomewater

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Area of Science:

  • Environmental microbiology
  • Molecular ecology
  • Water quality assessment

Background:

  • Traditional water quality testing methods have limitations in comprehensively characterizing microbial communities.
  • Next-generation sequencing (NGS) and DNA metabarcoding offer advanced tools for microbial analysis.
  • Previous studies highlight the utility of DNA metabarcoding for diverse drinking water sources.

Purpose of the Study:

  • To evaluate the application of DNA metabarcoding for characterizing bacterial microbiomes in private freshwater sources in Antigua and Barbuda.
  • To compare microbial diversity and composition across different water storage systems (well, tank, cistern).
  • To identify potentially pathogenic bacteria in these water sources.

Main Methods:

  • Collected replicate water samples from three private residential sources: a well, an above-ground tank, and a cistern.
  • Utilized 16S rRNA metabarcoding to assess bacterial microbiomes.
  • Analyzed alpha and beta diversity, performed taxonomic analysis, and screened for pathogenic species.

Main Results:

  • Significant differences in microbiome composition and relative abundance were observed between water sources.
  • The cistern exhibited lower alpha diversity compared to the well and tank.
  • Potentially pathogenic species or genera harboring them were detected in all tested water samples.

Conclusions:

  • DNA metabarcoding provides an effective and comprehensive assessment of drinking water quality.
  • This method can identify pathogenic species missed by traditional techniques.
  • The approach shows potential for tracing disease outbreaks and is novel for small Caribbean island nations.