An Automated Centrifugal Microfluidic Platform for Efficient Multistep Blood Sample Preparation and Clean-Up towards
Yuting Hou1, Rohit Mishra2,3, Yufeng Zhao1,4,5
1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Micromachines
|December 23, 2023
Summary
This study presents a microfluidic platform automating whole blood sample preparation for mass spectrometry. The centrifugal system efficiently cleans up samples in 35 minutes, improving small molecule detection.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Microfluidics
Background:
- Traditional mass spectrometry sample preparation is labor-intensive and prone to errors.
- Automating sample preparation is crucial for high-throughput analysis and reproducible results.
Purpose of the Study:
- To develop and demonstrate a centrifugal microfluidic platform for automated whole blood sample preparation.
- To integrate this platform with mass spectrometry techniques for small molecule detection.
Main Methods:
- Utilized a centrifugal microfluidic disc combining liquid-phase and solid-phase extractions.
- Employed methanol for protein precipitation and C18 beads/silica particles for lipid and protein removal.
- Integrated matrix-assisted laser desorption/ionization (MALDI) MS and hydrophilic interaction liquid chromatography (HILIC) MS for analysis.
Main Results:
- Successfully automated whole blood sample preparation and clean-up in 35 minutes.
- Demonstrated effective removal of proteins and lipids, and segregation of salts.
- Analyzed ionic metabolites, including amino acids and organic acids, using MALDI-MS and HILIC-MS.
Conclusions:
- The centrifugal microfluidic platform offers an efficient and automated solution for blood sample preparation for mass spectrometry.
- This technology enhances throughput and accuracy for small molecule detection in biological samples.
Keywords:
lab-on-a-discmass spectrometrymicrofluidicssmall molecule analysiswhole blood sample preparation
