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Updated: Jul 7, 2025

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
Published on: September 26, 2019
Composition and structure analysis of different depths in the stratum corneum using confocal Raman microscopy
Feng Zhong1, Feng Wang1, Haifeng Yang1
1The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China.
Abstract:
The chemical composition and structure of the stratum corneum (SC) play a crucial role in the skin barrier function. Therefore, accurately determining the SC thickness and studying the changes in lipid and keratin structure and distribution within it are key aspects of skin barrier research. Currently, there are limited analytical tools and data analysis methods available for real-time and online studies of SC composition and structural changes. In this study, we focus on depth as a perturbation and employ confocal Raman microscopy combined with moving-window two-dimensional correlation spectroscopy (MW2D) technique to investigate the SC thickness. Additionally, we employ confocal Raman microscopy combined with perturbation-correlation moving-window two-dimensional correlation spectroscopy (PCMW2D) to precisely characterize the stratification of the SC. Furthermore, the two-dimensional correlation spectroscopy (2DCOS) method is utilized to examine the content of various conformations in the keratin secondary structure within the SC, as well as the subtle interrelationships between lipid and keratin structures.
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