Planting gold nanoflower for harvesting reproducible SERS substrate

Surface-enhanced Raman scattering (SERS) is an ultrasensitive analytical method which has been applied in many fields, and the reproducibility of the substrate is important for reliable SERS analysis. In present work, an innovative method inspired by the flower planting process is put forward to acquire gold nanoflower (AuNF) SERS substrate. Three steps (digging holes, sowing the gold nanoseeds and seeds grow into gold nanoflowers) are included in the substrate fabrication process, and the influence of preparing conditions (like reacting time and Na₃Au(SO₃)₂ concentration) on the substrate morphology and SERS performance are investigated. The acquired AuNF substrate not only exhibits good SERS performance but also possesses excellent reproducibility while being used to detect the rhodamine 6G (R6G) molecular. The relative standard deviation (RSD) of Raman signals among substrates acquired in distinct batches (substrate-to-substrate) is as low as 6.67 %. Since the AuNF substrate is prepared by the wet chemistry route based on seed-mediated growth and there are no expensive reagents or complicated process used, the new process to obtain AuNF substrate is cost-effective and easy to scale up.