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Updated: Aug 4, 2026

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
Published on: January 22, 2015
Innovative determination of the specific anammox activity for anammox sludge from continuous flow reactors: A
Hao Xue1, Han Wang1, Mingda Zhou1
1State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
Abstract:
A novel method for measuring specific anammox activity (SAA) was proposed based on continuous flow tests to accurately determine the SAA of anammox sludge from continuous flow reactors, resolving the challenges of inaccurate SAA assessment caused by substrate shock to anammox bacteria. Results showed SAA of expanded granular sludge bed sludge via batch tests (0.101 ± 0.018 g-N·g-VSS-1·d-1) was lower than continuous flow tests (0.206 ± 0.010 g-N·g-VSS-1·d-1) (p < 0.05), highlighting the impact of substrate shock. Conversely, SAA of sequencing batch reactor sludge assessed via batch tests (0.878 ± 0.008 g-N·g-VSS-1·d-1) was higher than continuous flow tests (0.809 ± 0.005 g-N·g-VSS-1·d-1) (p < 0.01), attributed to endogenous denitrification. The advantages of continuous flow tests over batch tests included milder feeding way, stricter anaerobic conditions, and minimal sampling impact on system. Our study contributes to more accurate measurements of SAA of anammox sludge from continuous flow reactors, favoring long-term robust operation of anammox reactors.
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