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Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
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Related Experiment Video

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Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation
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Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

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Shuxuening Injection Inhibits Apoptosis and Reduces Myocardial Ischemia-Reperfusion Injury in Rats through PI3K/AKT

Tong-Tong Yue1, Ying-Jie Cao2, Ya-Xuan Cao1

  • 1The First Clinical Medical College of Henan University of Chinese Medicine, Zhengzhou, 450046, China.

Chinese Journal of Integrative Medicine
|December 28, 2023
PubMed
Summary

Shuxuening Injection (SXNI) effectively treats myocardial ischemia-reperfusion injury (MIRI) by activating the PI3K/AKT pathway. This treatment reduces heart damage and cardiomyocyte apoptosis in MIRI models, offering a potential therapeutic strategy.

Keywords:
Ginkgo biloba leavesPI3K/AKT signaling pathwayShuxuening Injectionapoptosismyocardial ischemia reperfusion injury

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Area of Science:

  • Pharmacology
  • Cardiovascular Research
  • Traditional Chinese Medicine

Background:

  • Myocardial ischemia-reperfusion injury (MIRI) remains a significant clinical challenge.
  • Shuxuening Injection (SXNI), derived from Ginkgo biloba leaves, is used in MIRI treatment.
  • Understanding SXNI's mechanism of action is crucial for optimizing its therapeutic application.

Purpose of the Study:

  • To elucidate the key components and therapeutic mechanisms of Shuxuening Injection (SXNI) in myocardial ischemia-reperfusion injury (MIRI).
  • To investigate the role of the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway in SXNI's protective effects.
  • To validate the efficacy of SXNI in an in vivo rat model of MIRI.

Main Methods:

  • Network pharmacology approach using databases like TCMSP and PharmMapper to identify active components and targets of SXNI.
  • Construction of active ingredient-target networks and pathway analysis (KEGG) using Cytoscape and Metascape.
  • In vivo validation in a rat MIRI model, assessing infarct size, pathological damage, apoptosis, and protein expression via biochemical and histological assays.

Main Results:

  • Network pharmacology identified key targets and pathways, highlighting the PI3K/AKT signaling pathway's involvement.
  • In vivo studies demonstrated that SXNI significantly reduced myocardial infarction area and cardiomyocyte apoptosis in MIRI rats.
  • SXNI treatment upregulated the expression of p-PI3K, p-AKT, and Bcl-2, while downregulating Bax and cleaved caspase 3, indicating an anti-apoptotic effect.

Conclusions:

  • Shuxuening Injection (SXNI) exerts protective effects against myocardial ischemia-reperfusion injury (MIRI).
  • The therapeutic mechanism involves the activation of the PI3K/AKT signaling pathway.
  • SXNI demonstrates significant potential as a treatment for MIRI.