Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

152.5K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
152.5K
Tight Junctions01:29

Tight Junctions

5.3K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.3K
Phase I Reactions: Reductive Reactions01:27

Phase I Reactions: Reductive Reactions

206
Phase I biotransformation reductive reactions are chemical processes that modify drugs by introducing or revealing polar functional groups via reduction. Enzymes called reductases catalyze these reactions, playing a pivotal role in drug metabolism by transforming lipophilic drugs into more polar, water-soluble metabolites for easy excretion. An essential type of reductive reaction is the carbonyl group reduction, where aldehydes and ketones are reduced to alcohols. An example is the...
206
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

1.9K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
1.9K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.0K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional screen for subtype specificity of voltage sensor-targeted Kv7 potentiators.

British journal of pharmacology·2026
Same author

Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivation.

iScience·2026
Same author

Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation.

Glia·2026
Same author

Peripheral immune response and axonal degeneration in the hind paw skin of mice with experimental autoimmune encephalomyelitis.

Neurobiology of pain (Cambridge, Mass.)·2026
Same author

Clinical Manifestations.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Clinical Manifestations.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same journal

mTORC2-Na<sub>v</sub>1.2 signaling drives early hyperexcitability in Alzheimer's disease mouse model.

Channels (Austin, Tex.)·2026
Same journal

Role for TREK-1 as a polymodal sensor and regulator of cell activity.

Channels (Austin, Tex.)·2026
Same journal

Quantitative analysis of trafficking defects induced by heterozygous expression of hERG voltage sensor domain variants.

Channels (Austin, Tex.)·2026
Same journal

Transient receptor potential canonical (TRPC) channels in diabetes and associated complications.

Channels (Austin, Tex.)·2026
Same journal

Transient receptor potential channels in <i>Flaviviridae</i> infection: A comprehensive review.

Channels (Austin, Tex.)·2026
Same journal

TRPV4 mediates macrophage polarization involved in inflammatory root resorption induced by mechanical pressure.

Channels (Austin, Tex.)·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2025

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
10:07

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Published on: January 7, 2019

7.8K

Reducing agents facilitate membrane patch seal integrity and longevity.

Damayantee Das1, Anson Wong1, Timothy N Friedman2

  • 1Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, Canada.

Channels (Austin, Tex.)
|December 28, 2023
PubMed
Summary
This summary is machine-generated.

Adding reducing agents like DTT and TCEP to the bath solution improves giga-ohm seal formation and stability during patch clamp recordings. This technique enhances electrophysiological recordings in various cell types.

Keywords:
DTTTCEPgiga-ohmintegritylongevitypatch clampqualityreducing agentsseals

More Related Videos

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.3K
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
09:03

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Published on: June 21, 2013

22.3K

Related Experiment Videos

Last Updated: Jul 6, 2025

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
10:07

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Published on: January 7, 2019

7.8K
Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.3K
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
09:03

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Published on: June 21, 2013

22.3K

Area of Science:

  • Electrophysiology
  • Cellular Biology
  • Biophysics

Background:

  • The patch clamp technique is crucial for studying ion channels and cellular activity.
  • High-quality recordings depend on forming a stable giga-ohm seal, which can be difficult to achieve.
  • Improving giga-ohm seal formation and longevity is essential for reliable electrophysiological data.

Purpose of the Study:

  • To investigate the effect of reducing agents on giga-ohm seal formation and stability.
  • To identify methods for enhancing the success rate of patch clamp recordings.
  • To provide a practical tool for improving electrophysiological data quality.

Main Methods:

  • Whole-cell patch clamp recordings were performed on heterologous cells (HEK, LM) and primary cultured cells (DRG neurons).
  • Reducing agents (DTT, TCEP) were added to the external bath solution.
  • The impact of reducing agents on giga-ohm seal formation and stability at hyperpolarizing voltages was assessed.
  • An oxidizing agent (H2O2) was used as a control.

Main Results:

  • Application of DTT and TCEP significantly enhanced giga-ohm seal formation.
  • Reducing agents prolonged the stability of the giga-ohm seal, especially under strong hyperpolarizing conditions.
  • The oxidizing agent H2O2 demonstrated an adverse effect on seal integrity.
  • Improved seal quality was observed across different cell types tested.

Conclusions:

  • Reducing agents are effective in promoting giga-ohm seal formation and stability in patch clamp experiments.
  • This method offers a valuable approach to improve the reliability and success rate of electrophysiological recordings.
  • The findings suggest a simple yet effective strategy for optimizing patch clamp data acquisition in various research settings.