Highly stable glucose oxidase polynanogel@MXene/chitosan electrochemical biosensor based on a multi-stable interface...

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biosensor Technology

Background:

• Enzyme electrochemical biosensors require high sensitivity and stability for practical applications.
• Improving enzyme stability and sensor performance is a key challenge in biosensor development.

Purpose of the Study:

• To construct a stable enzyme electrochemical biosensor with enhanced sensitivity and stability.
• To investigate the use of nanocomposites with multi-stable interface structures for biosensor applications.

Main Methods:

• Preparation of a nanocomposite (PGOx@MXene/CS) via electrostatic assembly of GOx polynanogel (PGOx) onto MXene nanosheets.
• Utilizing MXene's large surface area for efficient loading and PGOx for enzyme stabilization.
• Characterization of the nanocomposite and evaluation of the biosensor's performance for glucose detection.

Main Results:

• The glucose sensor exhibited a linear range of 0.03-16.5 mM, sensitivity of 48.98 μA mM⁻¹·cm⁻², and a detection limit of 3.1 μM.
• The biosensor maintained 85.83% of its initial current after 200 cycles, demonstrating excellent stability.
• The sensor showed high sensitivity, selectivity, and reproducibility.

Conclusions:

• The developed multi-stable enzyme electrochemical biosensor effectively enhances enzyme stability and sensor performance.
• The nanocomposite material provides a promising platform for stable and continuous blood glucose detection.
• This approach offers a viable solution for improving the reliability of enzyme-based biosensors.