Related Experiment Video
Updated: Jul 6, 2025
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Distribution of group a Rotavirus circulating in Mashhad, Iran
Kiana Ketabi1, Zahra Meshkat2, Hoorieh Soleimanjahi1
1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Insights
Group A Rotavirus (RVA) causes significant diarrheal disease in young children. This study found RVA in 31.3% of pediatric fecal samples in Mashhad, Iran, with higher prevalence in older infants.
Area of Science:
- Pediatrics
- Infectious Diseases
- Virology
Background:
- Group A Rotavirus (RVA) is a primary cause of acute diarrheal disease (ADD) in children under five.
- Understanding RVA epidemiology is crucial for public health strategies and vaccine development.
Purpose of the Study:
- To determine the distribution and prevalence of circulating RVA strains in Mashhad, Iran.
- To inform health improvement strategies and vaccine decision-making for pediatric populations.
Main Methods:
- Fecal specimens were collected from 106 children hospitalized with diarrhea in Mashhad.
- Samples negative for bacterial, parasitic, and amoebic infections were tested for RVA using RT-PCR.
Main Results:
- RVA was detected in 31.3% of the analyzed pediatric fecal specimens.
- No significant difference in RVA positivity was observed based on gender or season (fall/winter).
- RVA prevalence increased with age, particularly in children aged 1 to 60 months.
Conclusions:
- Rotavirus remains a significant cause of acute diarrheal disease in Mashhad's pediatric population.
- Further research into the genetic diversity of circulating RVA strains is warranted.
Background And Objectives:
Group A Rotavirus (RVA) is the most important causative agent of acute diarrheal disease in pediatrics 5 years and below. This study aimed to determine the distribution of circulating RVA in Mashhad, Iran to develop health improvement strategies and vaccine decision making.
Materials And Methods:
A total of 106 fecal specimens were collected from children admitted to Akbar and Dr. Sheikh referral pediatric hospitals of Mashhad City during the December 2020 to March 2021 and December 2021 to March 2022. All specimens were tested for specific bacterial, parasitic, and amoebic infections. Negative samples were analyzed for RVA infections using the RT-PCR method.
Results:
RVA was detected in 31.3% of the specimens, indicating no statistical significance in gender distribution or between fall and winter positivity rates. The number of RVA-positive specimens increased following age increasing in the range of 1 to 60 months.
Conclusion:
Today, acute diarrheal disease (ADD) is still caused mostly by Rotavirus infections in pediatrics in Mashhad. Comprehensive studies are needed to determine the genetic diversity of circulating Rotavirus strains in this era.
More Related Videos
15:16Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
12:32EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016