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Non-specific signals in real-time RT-PCR for detecting respiratory viruses
Gannon C K Mak1, Derek L L Hung1
1All from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, People's Republic of China.
Diagnostic Microbiology and Infectious Disease
|December 29, 2023
Abstract:
We describe our experiences in investigating the origin of non-specific signals during the development phase of a multiplex PCR assay for respiratory viruses. After ruling out various sources of error, eventually we discovered the non-specific signal was related to the particular lot of the PCR kit.

