Whole genome sequencing using amplicon-based and hybridization-based methods for respiratory syncytial virus

Area of Science:

• Virology
• Genomics
• Molecular Biology

Background:

• Whole genome sequencing (WGS) is crucial for understanding viral evolution and transmission.
• Multiple WGS protocols exist for respiratory syncytial virus (RSV).
• Direct comparisons of different WGS methodologies over extended periods are lacking.

Purpose of the Study:

• To compare the performance of amplicon-based and hybridization-based WGS methods for respiratory syncytial virus (RSV).
• To evaluate these methods across a 12-year collection of RSV strains.

Main Methods:

• Utilized established amplicon-based sequencing protocols.
• Employed hybridization-based sequencing techniques.
• Analyzed RSV strains collected over a 12-year timeframe.

Main Results:

• Observed significant differences in the performance of amplicon-based and hybridization-based sequencing methods.
• Performance varied across the RSV strains analyzed.
• Identified limitations and strengths of each method in capturing RSV genomic diversity.

Conclusions:

• The choice of WGS method impacts the sequencing outcomes for respiratory syncytial virus (RSV).
• Amplicon-based and hybridization-based approaches exhibit distinct performance characteristics for RSV.
• Further method optimization may be needed for comprehensive RSV genomic surveillance.