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Updated: Jul 6, 2025

Linearization of the Bradford Protein Assay
Published on: April 12, 2010
Comparison of the effect of Sodium Chloride concentration on protein determination: Bradford and Biuret methods
1Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, 81531-990, Curitiba, PR, Brazil.
Abstract:
Protein quantification methods using spectrophotometry are widely used in laboratory routines for different purposes. Samples generally contain non-protein components that can interfere with the quality of the analysis. A simple and quick test with different concentrations of sodium chloride demonstrated that the Bradford method is significantly affected by the presence of salt, while Biuret remains stable. Therefore, the choice of method is an important factor in reducing errors and ensuring more reliable results.
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