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Updated: Jul 6, 2025
Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination
Published on: July 4, 2007
Persistence of dengue genome in a remotely infected patient
Soraya Thaivanich1,2, Jirayu Visuthranukul1,2, Kesinee Arunyingmongkol1,2
1Clinical Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
Background:
Dengue virus infection is an intriguing illness. It is traditionally thought of as a self-limited and nonpersistent disease.
Objectives:
We report a case with persistent dengue virus genome detectable in hematopoietic cells of a person with remote infection.
Methods:
A patient with multiple myeloma in remission was prepared for peripheral blood stem cell (PBSC) transplantation. Plasma and G-CSF-stimulated, mobilized PBSCs were collected. Dengue-specific reverse transcription polymerase chain reaction (RT-PCR) was performed in both pre- and post-stimulated blood specimens. Anti-dengue antibodies by ELISA and by neutralization assay were measured before and after the stem cell mobilization.
Results:
The viral genome was detected only in the PBSC of the post-G-CSF-stimulated specimens. Anti-dengue antibodies were negative and positive, by ELISA and neutralization assays, respectively, both before and after stem cell mobilization.
Conclusion:
Our findings reveal a persistent infection. Whether and how this strain may interact with subsequent serotype(s) remains to be elucidated.
