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A gut microbiome signature for HIV and metabolic dysfunction-associated steatotic liver disease
Javier Martínez-Sanz1,2, Alba Talavera-Rodríguez1,2,3, Jorge Díaz-Álvarez1
1Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
Introduction:
Metabolic dysfunction-associated steatotic liver disease (MASLD), has emerged as an increasingly recognized problem among people living with HIV (PLWH). The gut-liver axis is considered to be strongly implicated in the pathogenesis of MASLD. We aimed to characterize the gut microbiota composition in PLWH and MASLD and compare it with that of two control groups: PLWH without MASLD and individuals with MASLD without HIV infection.
Methods:
We collected clinical data and stool samples from participants. Bacterial 16S rRNA genes were amplified, sequenced, and clustered into operational taxonomic unit. Alpha diversity was studied by Shannon and Simpson indexes. To study how different the gut microbiota composition is between the different groups, beta diversity estimation was evaluated by principal coordinate analysis (PCoA) using Bray-Curtis dissimilarity. To further analyze differences in microbiome composition we performed a linear discriminant analysis (LDA) effect size (LEfSe).
Results:
We included 30 HIV+MASLD+, 30 HIV+MASLD- and 20 HIV-MASLD+ participants. Major butyrate producers, including Faecalibacterium, Ruminococcus, and Lachnospira dominated the microbiota in all three groups. Shannon's and Simpson's diversity metrics were higher among MASLD+ individuals (Kruskal-Wallis p = 0.047). Beta diversity analysis showed distinct clustering in MASLD-, with MASLD+ participants overlapping regardless of HIV status (ADONIS significance <0.001). MASLD was associated with increased homogeneity across individuals, in contrast to that observed in the HIV+NAFDL- group, in which the dispersion was higher (Permanova test, p value <0.001; ANOSIM, p value <0.001). MASLD but not HIV determined a different microbiota structure (HIV+MASLD- vs. HIV+MASLD+, q-value = 0.002; HIV-MASLD+ vs. HIV+MASLD+, q-value = 0.930; and HIV-MASLD+ vs. HIV+MASLD-, q-value < 0.001). The most abundant genera in MASLD- were Prevotella, Bacteroides, Dialister, Acidaminococcos, Alloprevotella, and Catenibacterium. In contrast, the most enriched genera in MASLD+ were Ruminococcus, Streptococcus, Holdemanella, Blautia, and Lactobacillus.
Conclusions:
We found a microbiome signature linked to MASLD, which had a greater influence on the overall structure of the gut microbiota than HIV status alone.
Insights
Metabolic dysfunction-associated steatotic liver disease (MASLD) significantly alters gut microbiota composition in people living with HIV (PLWH). MASLD, not HIV, drives these microbiome changes, impacting gut health.
Area of Science:
- Microbiology
- Hepatology
- Infectious Diseases
Background:
- Metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing concern for people living with HIV (PLWH).
- The gut-liver axis plays a crucial role in MASLD development.
- Understanding gut microbiota changes is key to addressing MASLD in PLWH.
Purpose of the Study:
- To characterize and compare gut microbiota composition in PLWH with MASLD.
- To compare gut microbiota in PLWH with MASLD to PLWH without MASLD and individuals with MASLD without HIV.
Main Methods:
- Collected clinical data and stool samples.
- Performed 16S rRNA gene sequencing for bacterial analysis.
- Utilized alpha and beta diversity metrics (Shannon, Simpson, PCoA, Bray-Curtis) and LEfSe for statistical analysis.
Main Results:
- MASLD was associated with higher gut microbial diversity (Shannon, Simpson).
- Beta diversity analysis revealed distinct clustering based on MASLD status, irrespective of HIV.
- MASLD, not HIV, significantly altered gut microbiota structure and composition.
Conclusions:
- A distinct gut microbiome signature is associated with MASLD.
- MASLD has a more profound impact on gut microbiota structure than HIV status alone.
- These findings highlight the importance of the gut-liver axis in MASLD pathogenesis among PLWH.
