Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunogenicity Assessment for siRNA Therapeutics: A Risk-Based Proposal for Event-Driven Testing.

The AAPS journal·2026
Same author

External Fields as Control Strategies to Promote Biomolecule Nucleation.

Advances in biochemical engineering/biotechnology·2026
Same author

Corrigendum to 'Advances in the Basic Sciences in Thoracic Oncology in the Last 20 Years and Their Translational Impact' [Journal of Thoracic Oncology Volume 21 Issue 1 (2026) 41-76].

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2026
Same author

Investigating the Utility of Explainable Artificial Intelligence for Neuroimaging-Based Dementia Diagnosis and Prognosis.

Human brain mapping·2026
Same author

Immunohistochemical biomarker-associated radiomics for classifying thymic epithelial tumors: a multicenter retrospective study.

NPJ precision oncology·2026
Same author

Advances in the Basic Sciences in Thoracic Oncology in the Last 20 Years and Their Translational Impact.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2026
Same journal

Novel Parent Survey Measures Sensory Behaviors Incorporating Sensory Modality and Stimulus Intensity.

Heliyon·2026
Same journal

Expression of concern: "SQSTM1/p62 promotes the progression of gastric cancer through epithelial-mesenchymal transition" [Heliyon 10 (2024) e24409].

Heliyon·2026
Same journal

Expression of concern: "TL1A promotes metastasis and EMT process of colorectal cancer" [Heliyon 10 (2024) e24392].

Heliyon·2026
Same journal

Expression of concern: "Factors affecting timing of surgery following neoadjuvant chemoradiation for esophageal cancer" [Heliyon 9 (2023) e23212].

Heliyon·2026
Same journal

Expression of concern: "On stratified single-valued soft topogenous structures" [Heliyon 10 (2024) e27926].

Heliyon·2026
Same journal

Expression of concern: "Artifact removal and motor imagery classification in EEG using advanced algorithms and modified DNN" [Heliyon 10 (2024) e27198].

Heliyon·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

525

Biocompatible optical physically unclonable function hydrogel microparticles for on-dose authentication.

Mengmeng Zhang1, Aparna Raghunath1, An Zhao1

  • 1Process & Energy Department, Delft University of Technology, Leeghwaterstraat 39, 2628 CB Delft, the Netherlands.

Heliyon
|January 1, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces on-dose Physical Unclonable Functions (PUF) for pharmaceutical authentication, embedding microspheres in hydrogels to create unique drug fingerprints. This novel method allows patients to verify medications using mobile phones, enhancing drug security and preventing counterfeits.

Keywords:
Anti-counterfeitingBiocompatibleColloidEmulsionHydrogelOn-dose authenticationOptical PUFPhysical unclonable function

More Related Videos

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.2K
Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

12.1K

Related Experiment Videos

Last Updated: Jul 6, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

525
An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.2K
Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

12.1K

Area of Science:

  • Pharmaceutical Sciences
  • Materials Science
  • Cryptography

Background:

  • On-dose authentication (ODA) uses unique tags to prevent counterfeit drugs.
  • Graphical Physical Unclonable Functions (PUF) offer high security but have limitations in pharmaceutical applications due to toxicity and specific imaging needs.
  • Existing methods struggle with non-replication and reverse engineering challenges.

Purpose of the Study:

  • To develop a novel on-dose PUF system for pharmaceutical authentication.
  • To create a secure, non-toxic, and easily implementable method for verifying medication authenticity.
  • To enable patients to authenticate their drugs using accessible technology like mobile phones.

Main Methods:

  • Embedding microspheres (superparamagnetic colloids or oil drops) randomly within biocompatible hydrogel particles to create unique drug fingerprints.
  • Utilizing microsphere diameter and coordinates as input for cryptographic key generation.
  • Employing a universal circle identification and binning program for data extraction.
  • Validating the system using white light illumination and low-magnification microscopy.

Main Results:

  • Demonstrated two types of on-dose PUFs: one with superparamagnetic colloids and another with sunflower oil drops.
  • The single-diameter system successfully passed NIST Statistical tests, showing sufficient randomness, bit uniformity, and device uniqueness.
  • The system enables authentication via mobile phones, requiring only white light and low-magnification imaging.
  • The single-diameter system exhibited an encoding capacity of , indicating significant labeling potential.

Conclusions:

  • The proposed on-dose PUF method provides a secure, user-friendly, and cost-effective solution for pharmaceutical authentication.
  • This technology overcomes limitations of current graphical PUFs, making it suitable for widespread pharmaceutical use.
  • On-dose PUFs have the potential to significantly enhance drug security, protect patients from counterfeit medications, and improve supply chain integrity.