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Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

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RNA fusion in human retinal development.

Wen Wang¹, Xiao Zhang¹, Ning Zhao¹

¹Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

|January 2, 2024

Summary

This summary is machine-generated.

Chimeric RNAs are present in human retinal cells and influence retinal development. Their downregulation disrupts retinal cell differentiation, impacting eye development.

Keywords:

chimeric RNA developmental biology human neuroscience retinal development transcriptome

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Area of Science:

Genetics
Developmental Biology
Ophthalmology

Background:

Chimeric RNAs are found in human cells and regulate stem cell functions and neural development.
Their presence and role in human retinal development are currently unknown.

Purpose of the Study:

To investigate the expression atlas of chimeric RNAs during human retinal development.
To determine the physiological functions of chimeric RNAs in the developing human retina.

Main Methods:

Utilized human embryonic stem cell-derived retinal organoids (ROs) from day 0 to 120.
Analyzed chimeric RNA expression patterns and performed loss-of-function studies on a specific chimeric RNA (CTCL).

Main Results:

Identified common and novel chimeric RNAs during retinal development.
Confirmed universal presence of CTNNBIP1-CLSTN1 (CTCL) chimeric RNA in human retinas and retinal organoids.
Demonstrated that CTCL downregulation leads to precocious neuronal differentiation and a shift in progenitor cell fate towards retinal pigment epithelium at the expense of retinal cells.

Conclusions:

Chimeric RNAs play a critical role in human retinal development.
CTCL is essential for proper retinal cell differentiation and fate determination.